【摘要】 采用沉淀法合成了以OH-、F-、Cl-为通道离子的磷灰石纳米晶体。利用其独特的多位点吸附特性,研究了磷灰石不同通道离子替换对细胞色素C直接电化学的影响。在pH7.0的磷酸盐缓冲溶液中,细胞色素C在磷灰石修饰玻碳电极表面于0.074V(vs.Ag/AgCl)附近有一对准可逆的氧化还原峰,为细胞色素C血红素辅基Fe/Fe电对的特征峰。细胞色素C与磷灰石之间的静电作用使深藏在细胞色素C内部的电活性中心靠近电极表面,加速了细胞色素C在玻碳电极表面扩散控制的准可逆单电子转移过程。其中氟磷灰石对细胞色素C直接电化学的促进作用最显著,羟基磷灰石次之,氯磷灰石最弱。更多还原

【Abstract】 The nano-scale apatites of OH-、F-、Cl-channel-ions substitution were prepared with precipitation method. Due to the multi-adsorbing sites of apatites, the influence of apatite channel-ions substitution on the direct electrochemical of cyctochrome C (Cyt C) was investigated. Cyclic voltammograms of Cyt C on the ~apatite modified glassy carbon electrodes show a pair of well-defined and nearly reversible peaks at about ~0.074 V (vs.Ag/AgCl) in pH 7.0 phosphat buffer solution, being the characteristic peaks of the Cyt C heme ~Fe/Fe redox couples. The experimental results support that the electrostatic interaction between Cyt C and ~apatite gives a rapid one-electron diffusion-controlled electron transfer. The P sites of negative charges on apatite attached to the positive charges of the lysine residues, which made the heme sites of Cyt C take ~effective orientation and allowed the direct electron transfer to take place. The promotion of fluorapatite for the electron transfer of Cyt C was the most remarkable one and the chlorapatite was the weakest. This derived from the ~different electrostatic interaction between apatite and Cyt C.更多还原