【摘要】 目的 :探讨乏凝血因子V血浆制备方法及凝血因子V活性 (FV C)测定病毒性肝炎患者中的临床意义。方法 :采用温育法制备乏因子V基质血浆并应用于临床检测。病毒性肝炎住院病人 175例 ,分急性肝炎、慢性轻度肝炎、慢性中 /重度肝炎、肝硬化和重症肝炎五个组 ,检测血浆中FV C ,并与 36例正常对照组测定结果相比较。结果 :自制乏因子V基质血浆检测结果 :与正常对照组比较 ,除急性肝炎外 ,慢性轻度肝炎轻度异常 (P <0 0 5 ) ,慢性中 /重度肝炎、重症肝炎和肝硬化患者血浆中FV C则明显下降 (P <0 0 0 1) ,差异均有显著意义。结论 :自制乏因子V血浆 ,简单易行 ,结果满意。FV C的下降与病情严重程度相关 ,该测定对于判断重症肝炎病情和观察其预后有重要的临床价值。更多还原

【Abstract】 Objective To develop a practical method of preparing coagulation factor V deficient plasma for testing factor V coagulant activity (FVC) clinically in patients with viral hepatitis. Methods Coagulation factor V deficient plasma was prepared from pooled normal plasma with incubation at 37℃ until the protbrombin time of the specimen was over 60 seconds. Factor V coagulant activity (FVC) was tested with this preparation in 175 patients with viral hepatitis of different types and 36 controls. Results Except in patients with acute hepatitis (n=76), the FVC (%) decreased significantly in all other types of patients (P<0.05 in mild chronic hepatitis (n=25); P<0.001 in moderate to severe chronic bepatitis (n=36)、 severe acute hepatitis (n=15) and cirrbosis (n=23). Conclusion Prepartion of coagulation factor V deficient plasma with this method is easy and practical with satisfactory result. Determination of FVC (%) in patients with viral hepatitis is clinically useful for judgement of the severity of the disease as well as outcome prediction.更多还原