【摘要】 目的 :研究S - 10 0、CD80和CD86在脑胶质瘤中的表达及其生物学意义。方法 :应用免疫组织化学染色法检测 30例脑胶质瘤、10例瘤旁组织和 10例正常脑组织手术标本中S - 10 0、CD80和CD86的表达水平。结果 :S - 10 0蛋白在脑胶质瘤、瘤旁组织和正常脑组织的阳性率均为 10 0 % ,用HPISA - 10 0 0图像分析测得其不同的平均阳性灰度值 (AGV)分别为 72 2 0± 12 30、35 6 0± 9 5 0和 2 8 6 0± 10 2 0 ,脑胶质瘤组织与正常脑组织比较 ,差别有统计学意义 (P <0 0 5 ) ,而瘤旁组织与正常脑组织比较 ,差别无统计学意义 (P >0 0 5 )。脑胶质瘤瘤旁组织及正常脑组织中未发现CD80和CD86表达 ,脑胶质瘤组织未发现表达CD80 ,但有 1例 (1/ 30 )CD86弱阳性。结论 :S - 10 0蛋白在胶质瘤组织中表达上调提示与胶质细胞的异常增殖及胶质瘤的发生发展有关。CD80和CD86在正常脑组织及胶质瘤中到底是否表达尚待进一步深入研究。更多还原

【Abstract】 Objective To explore the expression of S-100 protein, CD80 and CD86 in human glioma and its biological significance. Methods The expressions of S-100, CD80 and CD86 were observed in 30 surgical pathologic sections of human glioma tissue, 10 sections of paraglioma tissue and 10 sections of normal brain tissue with immunohistochemical staining technique. Results The positive rates of S-100 were 100% in all these three groups, the AGV (Average Positive Gray Scale Value) of positive stain were 72.20±12.30, 35.60±9.50 and 28.60±10.20 respectively in human glioma tissues, paraglioma tissues and normal brain tissues measured by analysis system of type HPISA-1000. The difference of AGV between glioma tissues and normal brain tissues was statistically significant (P<0.05), but there was no significant difference between AGV of normal brain tissues and paraglioma tissues (P>0.05). No expressions of CD80 and CD86 were detected in paraglioma tissues and normal brain tissues. No exprossion of CD80 was detected in glioma tissue; only one section of glioma tissue was weakly postive of CD86. Conclusion The up-regulation expression of S-100 in human glioma indicates that S-100 protein is closely related to gliocyte proliferation and the pathogenesis of glioma. For expression of CD80 and CD86 in nomal brain and glioma tissue, further study is required.更多还原