【摘要】 目的　研究缺血预处理 (IP)对肝缺血再灌注 (IR)损伤后早期细胞增殖的影响。　方法　 5 4只SD大鼠随机分成IR组和IP组。利用肝原位部分IR模型 ,于复灌后 0 ,1,2 ,4h取材 ,制备单细胞悬液 ,应用流式细胞仪 ,以Ki 6 7抗原作为细胞增殖的定量分析方法 ,检测肝IR损伤后早期细胞增殖的变化 ,并观察IP对其影响。　结果　与IR组相比 ,在复灌后 0 ,1h ,IP组肝细胞Ki 6 7表达率明显增高 [(2 8 86± 6 34)vs (19 4 0± 5 35 ) ,(4 6 82± 9 80 )vs (2 2 4 0± 5 0 8) ,P <0 0 5 ],复灌后 2 ,4hIP组肝细胞Ki 6 7表达率变化不显著 [(35 77± 6 87)vs (34 2 1± 6 34) ,(4 2 0 8± 10 75 )vs (4 3 87± 10 77) ,P >0 0 5 ) ]。　结论　IP可促进肝细胞在IR损伤后早期细胞增殖 ,可能是其对IR损伤起到保护作用的机制之一。更多还原

【Abstract】 Objective To observe the effect of ischemic preconditioning on the rats liver cell proliferation after ischemia/reperfusion injury. Methods Fifty-four SD rats were randomly divided into ischemic preconditioning group(IP), ischemia/reperfusion group(IR) and sham operation group(SO). The partial liver ischemia/reperfusion model was performed. The liver cell was collected at 0, 1, 2, 4 h after ischemia/reperfusion. Cell proliferation were detected by Ki-67 antigen method with flow cytometry. Results Compared with IR group, IP group showed more Ki-67 positive cell at 0, 1 h after ischemia/reperfusion[(19.40±5.35) vs (28.86±6.34), (22.40±5.08) vs (46.82±9.80),P<0.05]. No difference between the two groups was found at 2, 4 h after reperfusion[(34.21±6.34) vs (35.77±6.87), (43.87±10.77) vs (42.08±10.75),P>0.05]. Conclusion Ischemic preconditioning before hepatic ischemia/reperfusion could promote cell proliferation in the early stage of reperfusion, which was associated with the hepatoprotective affects of ischemic preconditioning.更多还原