【摘要】 目的　观察引导组织再生 (GTR)术后不同时间龈沟液和牙槽骨中糖氨多糖 (GAG)变化 ,探讨龈沟液中 GAG变化对判断牙周组织再生成熟度有否帮助。　方法　将 6只杂种犬的下颌双侧第二、三、四前磨牙制备慢性 度根分叉病变模型 ,采用自身对照。对照组行常规翻瓣术 ,实验组行 GTR治疗 ,分别于术前和术后第 4 ,6 ,8周检测龈沟液及牙槽骨中 GAG水平。　结果　 GTR组术后第 4 ,6周龈沟液及牙槽骨中 GAG与基线水平比较差异有显著性。　结论　观察 GTR治疗术前后龈沟液中 GAG水平对判断牙周组织再生成熟度有一定帮助更多还原

【Abstract】 Objective To monitor glycosaminoglycans(GAG) in gingival crevicular fluid(GCF) and alveolar bone of mongrel dogs with periodontal class Ⅱ furcation involvement before and after guided tissue regeneration(GTR), and to investigate whether the levels of GAG can be used as a potential indicator of maturation of regenerative periodontium. Methods Class Ⅱ furcation involvement were surgically created in the 2, 3, 4th mandibular premolars in six mongrel dogs. 6 weeks later, GTR therapy and flap operation therapy was performed. GCF samples were taken at immediately prior to operation and at 4, 6, 8 weeks after surgery. Alveolar bone samples were taken at 4, 6, 8 weeks after surgery. GAG level was determined by using an Alcian blue dye method. Results In GTR groups, the levels of GAG in GCF samples and in alveolar bone samples were significantly increased at 4 and 6 weeks after surgery. Conclusion GAG in GCF may be a potential indicator of monitoring the status of regenerative periodontium.;更多还原