【摘要】 研究了福建沿海两种常见贝类波纹巴非蛤(Paphiaundulata)和菲律宾蛤仔(Ruditapesphilip-pinarum)的性腺和斧足的DNA提取方法,并且对它们进行了RAPD分析,结果显示性腺DNA得率较斧足高,并且成功获得了效果较好的2种随机引物及其相应的反应条件,能够在分子水平上为它们及其近源种的比较研究提供初步的资料.更多还原

【Abstract】 Researched on two shellfishes of Paphia (Neotapes) undulata and Ruditapes philippinarum by the molecular level, including the method of genomic DNA isolation and RAPD detection. The result suggested that the yield of DNA from gonad is higher than from pelecypodium. Moreover,two primers had been selected to identify these two shellfishes satisfactorily by RAPD analysis of the extracted genetic DNA. This research provided the basic molecular foundation for further identification of the shellfishes on molecular level.更多还原