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利用回交和MAS技术改良珍汕97B的白叶枯病抗性

Application of  backcrossing and marker-assisted selection to improve bacterial blight disease resistance of Zhenshan 97B

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【作者】 林荔辉陈志伟张积森吴为人周元昌唐定中

【Author】 LIN Li-hui~1, CHEN Zhi-wei~1, ZHANG Ji-sen~1, WU Wei-ren~(1,2), ZHOU Yuan-chang~1, TANG Ding-zhong~1 (1.College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, China)

【机构】 福建农林大学作物科学学院福建农林大学作物科学学院 福建福州350002福建福州350002福建福州350002浙江大学农业与生物技术学院浙江杭州310029福建福州350002

【摘要】 以IRBB21为白叶枯抗病基因的供体亲本,珍汕97B为受体亲本,通过1次杂交、3次回交、1次自交,采用传统的回交育种和分子标记辅助选择技术相结合的方法获得了6个导入Xa21的珍汕97B导入系,其遗传背景恢复比例为92.8%-97.4%(平均为94.9%).

【Abstract】 Using IRBB21 as the donor of the resistant gene to rice bacterial blight disease, resistant gene Xa21 was introgressed into elite variety Zhenshan 97B through one cross, three backcrosses and one selfing. Six near-isogenic lines of Zhenshan 97B with introgressed Xa21 were obtained through traditional breeding method combined with marker assisted selection, of which the recovery rates of Zhenshan 97B genetic background ranged from 92.8% to 97.4% (94.9% on average).

【基金】 国家高技术研究发展计划资助项目(2004AA211141);福建省重大科技项目资助项目(2000Z026);福建省自然科学基金资助项目(B9910011).
  • 【分类号】S511
  • 【被引频次】28
  • 【下载频次】209
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