【摘要】 本研究探讨了多重PCR技术在SARS病毒检测中的应用。根据香港中文大学在GenBank上公开发表的SARS病毒基因组cDNA序列,人工合成克隆特异性靶基因DNA片段,以此片段作为阳性样品,根据世界卫生组织推荐的进行单PCR与多重PCR检测分析。以单PCR法获得了121bp、182bp及302bp的靶基因片段3条;以二重PCR法获得了121bp+182bp、121bp+302bp与182b+302bp的靶基因片段组合;以二重PCR法获得了121bp+182bp+302bp的靶基因片段组合。结果表明:多重PCR技术可成功应用于SARS病毒的检测。更多还原

【Abstract】 The multiplex polymerase chain reaction (PCR) technique was applied to detect the Severe Acute Respiratory Syndrome (SARS) virus in present study. The target cDNA fragment of SARS virus synthesized artificially according to the genomic sequence of SARS submitted by Chinese University of Hong Kong to GenBank were used as positive samples. 5 primers recommended by WHO were used to amplify by single PCR and multiplex PCR. 3 target DNA fragments (121bp, 182bp and 302bp) were amplified by single PCR, as well as 3 associations of two target fragments including 121bp & 182bp. 121bp & 302bp and 182bp & 302bp, and the association of 3 target DNA fragments (121bp, 182bp and 302bp) were amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS virus, successfully.更多还原