【摘要】 利用大肠杆菌高效表达系统表达大豆Kunitz型胰蛋白酶抑制剂 (SBTi A2 )Tid,并利用亲和层析的方法纯化得到有抑制胰蛋白酶活性的SBTid.对重组SBTid的 pH稳定性和温度稳定性与天然SBTia进行了比较研究 .结果表明重组SBTid在高 pH下具有更好的稳定性 ,预示了SBTid作为生物农药或利用转基因方法提高作物抗虫性的应用前景更多还原

【Abstract】 One kind of Soybean kuntiz-type trypsin inhibitors（SBTi）-Ti^d was expressed in E.coli and purified by affinity chromatograph. The recombinant Ti^d showed trypsin inhibitory activity in vitro . The pH stability and thermal stability of Ti^a and recombinant Ti^d were studied. Compared with the natural SBTi^a,SBTi^d exhibited a better stability in a high pH spectrum and a similar thermal stability at less than 50 ℃ .These data suggested that SBTi^d has a promising application potential as a bio-pesticide and insect-resistant gene in crop designing.更多还原