【摘要】 目的　探讨甲醛致 DNA-蛋白质的交联作用以及交联的检测方法。方法　以人血淋巴细胞为材料 ,设定 0、0 .0 0 5、0 .0 2 5、0 .1 2 5、0 .6 2 5 mmol/ L 五个甲醛浓度梯度 ,采用 KCl- SDS沉淀法来检测染毒后细胞中 DNA-蛋白质交联的含量。结果　甲醛浓度在 0、0 .0 0 5、0 .0 2 5 mm ol/ L 时 ,交联现象不明显或不能诱导DPC(P>0 .0 5 ) ;在 0 .1 2 5、0 .6 2 5 mm ol/ L 时交联程度显著增加 (P<0 .0 1 ) ,并有较好的剂量 -效应关系。结论　在低浓度时 ,甲醛诱导 DNA-蛋白质交联现象不明显或不诱导该现象 ,在高浓度时可显著诱导交联现象。KCl- SDS沉淀法检测 DNA-蛋白质交联的含量是有效的更多还原

【Abstract】 Objective To explore the action of formaldehyde inducing DNA protein crosslinks(DPC) and to test the detection method. Methods This study took human blood lymphocyte as experimental material, chose 0、0 005、0 025、0 125、0 625 mmol/L five formaldehyde concentrations, and used KCl SDS method to detect DPC. Results At low concentrations(0 005, 0 025 mmol/L) formaldehyde cold not induce DPC(P>0 05); but at high concentrations(0 125, 0 625 mmol/L), it could induce DPC significantly(P<0 01), and there was good dose dependent relation. Conclusion At low concentrations formaldehyde could not induce DPC; but at high concentrations, it could induce DPC significantly. KCl SDS method was effective.更多还原