【摘要】 目的　探讨一种新的高灵敏度血清 HBV DNA水平检测方法以及病毒 DNA检测用于献血员筛选的可行性。方法　采用 Am pli Sensor定量荧光 PCR技术定量检测经 EL ISA法筛选的 32 1名健康志愿者和37名 HBs Ag携带者血清 HBV DNA水平。结果　 32 1名志愿者中 2名 HBV DNA阳性 (0 .6 % ) ;37例 HBs Ag携带者 DNA检出率 1 0 0 % ,但 DNA含量相差较大 (7.98± 5 .37)。结论　 Ampli Sensor定量荧光 PCR技术灵敏度可达对数值 1拷贝 /毫升 ,该法用于献血员病毒 DNA检测有一定意义 ,但更适合用于临床健康检查 ;同时发现 HBV在无症状 HBs Ag携带者体内病毒复制差异较大 ,可能与不同个体的免疫状况有关。更多还原

【Abstract】 Objective The application of a highly sensitive method AmpliSensor Assay for detection of hepatitis B virus(HBV) in sera of blood donors and asym ptomatic carriers was evaluated.Methods 321 volunteers and 37 HBV HBsAg healthy carriers,were determined by ELISA methods.Results 2 individuals among 321 volunteers were positive(0.6%)by fluorescent quantitative PCR technique.HBV DNA were found in all 37 healthy carriers(HBsAg positive).It was coincident with ELISA method.But the HBV DNA level was much diverse(7.98±5.37).Conclusion This fluorescent quantitative PCR system could detet the amount of HBV DNA as little as 1 copy/ml(Log). It is suggested that it may be used for screening blood donors. The results also show that HBV DNA detection is useful for early monitoring of HBV in clinical examination. The data also suggest that HBV exists in quite different ways in asymptomatic carriers, this may be related to the different immune status of individuals.更多还原