【摘要】 目的　探讨多药耐药因子在肺癌中表达与共表达的水平、相关性及临床意义。方法　采用免疫组化SABC技术检测 60例肺癌患者病理组织中P 糖蛋白 (P glycoprotein ,P gp)、多药耐药相关蛋白 (mul tidrug resistance associatedprotein ,MRP)、肺耐药蛋白 (lungresistanceprotein ,LRP)、谷胱甘肽 S转移酶π(GlutathioneS transferase ,GST π)等多药耐药因子的表达水平。结果　①P gp、MRP、LRP、GST π阳性表达率分别为 5 3 .3 %( 3 2 /60 ) ,63 .3 %( 3 8/60 ) ,70 .0 %( 4 2 /60 ) ,80 .0 %( 4 8/60 )。②耐药因子在不同病理类型中表达有显著性差异 (P <0 .0 5 ) ,在NSCLC中表达高于SCLC ;在不同TNM分期、不同分化程度间表达无显著性差异 (P >0 .0 5 )。③各耐药因子之间阳性共表达的结果分别为P gp +MRP :41.6%,P gp +LRP :3 5 .0 %,MRP +LRP :5 3 .3 %,MRP +GST π :5 0 .0 %,LRP +GST π :5 8.3 %,P gp +GST π :45 .0 %,P gp +MRP +LRP+GST π :2 0 .0 %。其中P gp与MRP间具有相关性 (rs=0 .75 6,P <0 .0 1) ,P gp与LRP间具有相关性 (rs=0 .689,P <0 .0 1) ,MRP与LRP间具有相关性 (rs=0 .669,P <0 .0 1) ,MRP与GST π间具有相关性 (rs=0 .5 46,P <0 .0 1) ,LRP与GST π间具有相关性 (rs=0 .848,P <0更多还原

【Abstract】 Objective To study the levels of expression, coexpression, and clinical significancer of four multidrug resistance factors in lung cancer. Methods The P glycoprotein (P gp), multidrug resistance associated protein (MRP), lung resistance protein (LRP), glutathione S transferase (GST π) of 60 lung cancer patients were detected by using immunohistochemical method. Results The positive rate of the drug resistance factor was 53.3% (32/60), 63.3% (38/60), 70.0% (42/60), and 80.0% (48/60) for P gp, MRP, LRP, and GST π respectively. Patients with NSCLC had significantly higher expression of the drug resistance factors than those with SCLC. On the other hand, no relationship was observed between the expression of drug resistance factors and TNM stage and cell differentiation. The coexpression rate was as follows: P gp+MRP, 41.6% ; P gp+LRP, 35.0%; MRP+LRP, 53.3%; MRP+GST π, 50.0%; LRP+GST π, 58.3%; P gp+GST π, 45.0%; P gp+MRP+LRP+GST π, 20.0%. Among them, a relationship was detected between P gp and MRP ( r s =0.756, P <0.01), between P gp and LRP ( r s =0.686, P <0.01), between MRP and LRP ( r s =0.669, P <0.01), between MRP and GST π( r s =0.546, P <0.01), between LRP and GST π ( r s =0.848, P <0.01), between P gp and LRP ( r s =0.689, P <0.01), and between P gp and GST π ( r s = 0.535 , P <0.01). Conclusion The MDR in lung cancer patients is affected by various multidrug resistance factors. The drug resistance factors’ expression is related to histology, but not to TNM stage and cell differentiation.更多还原