【摘要】 【目的】　探讨早期丰富环境对脑白质损害 (whitematterdamage ,WMD)新生大鼠脑发育的影响。　【方法】　选用 2日龄新生大鼠 3 4只 ,随机分为正常对照组 (共 9只 )、干预组 (共 12只 )及非干预组 (共 13只 ) ,后两组通过结扎右侧颈总动脉 ,吸入氧体积浓度为 ( 6.0± 0 .5 ) %的氮氧混合气 ,制作新生大鼠WMD模型 ,干预组采用早期触摸和丰富环境进行干预 ,总干预时间为 2 7d。干预结束后进行感觉运动功能、情感行为及学习记忆能力检测 ,以判断干预效果。并通过电镜观察超微结构改变。　【结果】　非干预组感觉运动功能、情感行为能力和学习记忆能力较正常对照组有不同程度减退 (P <0 .0 5 )。干预组上述能力均较非干预组有明显改善 (P <0 .0 1)。悬吊试验干预组得分 :4.0± 1.3 ,非干预组 1.6± 0 .7,正常对照组 3 .4± 0 .9;斜坡试验得分分别为 :2 .3± 1.4、7.1± 2 .4、 3 .2± 1.4;旷场试验得分分别为 :10 .3± 2 .7、4.6± 2 .7、12 .0± 3 .8;拒俘反应性得分分别为 :1.7± 1.6、0 .5± 0 .5、3 .3± 0 .6。避暗试验潜伏期得分分别为 :195 .2± 76.1、10 7.8± 5 2 .7、2 0 5 .0± 67.1;避暗试验错误次数分别为 :1.1± 1.0、3 .4± 1.6、1.3± 1.1;非干预组患侧海马CA1区髓鞘较正常对照组明显变薄 ,更多还原

【Abstract】 To study the influence of early enriched environment on brain development of white matter damage (WMD) in neonatal rats for which Han model was performed. Two days old postnatal SD rats ( n =34) were used and randomly divided into normal ( n =9), intervention ( n =12) and non intervention groups ( n =13). The latter 2 groups were subjected WMD at first: the right common carotid artery was dissected and ligated. After 2 h of recovery, the lotters were exposed to 6%oxygen 94% nitrogen gas mixture for 4 h. After that, intervention group received the neonatal handling and was kept in an enriched environment for 27 days. The abilities of sensorimotor movement (hanging test and inclined plane test), emotional behavior (open field test and resistant to capture test), learning and memory function (step though test) were observed till one month old, and Electron Microscope (EM) technique were used to examine the brain’s ultrastructure. The sensorimotor movement abilities , emotional behavior abilities, learning and memory function of non intervention group were much lower than those of normal control group and intervention group( P <0.05). Those abilities of intervention group were improved compared with non intervention group( P <0.01). In the hanging test, the scores were 4.0±1.3,1.6±0.7,3.4±0.9 for intervention, non intervention and normal groups, respectively. In the inclined plane test, the scores were 2.3±1.4,7.1±2.4,3.2±1.4, respectively. In the open field test, the scores were 10.3±2.7,4.6±2.7,12.0±3.8, respectively. In the resistant to capture test, the scores were 1.7±1.6,0.5±0.5,3.3±0.6, respectively. The escape latencies of step though test were 195.2±76.1,107.8±52.7, 205.0±67.1, respectively. The mistakes of step though test were 1.1±1.0, 3.4±1.6, 1.3±1.1, respectively. It could be seen from EM that the myelin sheath in hippocampus CA1 of non intervention group was much thin than those of normal group, it showed that WMD has disrupted myelination, and the myelin sheath of intervention group was much thick than those of non intervention group, but it was not as good as those of normal group. [Conclusion] Early enriched environment can improved brain development of WMD in neonatal rats which Han model has performed.更多还原