【摘要】 取老年猫 (12龄 ,3～ 3 5kg)和青年猫 (1～ 3龄 ,2～ 2 5kg)各 4只的视网膜 ,经 4 %多聚甲醛处理后 ,用H .E 染色以显示视网膜结构 ,Nissl染色显示神经节细胞 ,免疫组织化学ABC法染色以显示星形胶质细胞特征性标志物胶质纤维酸性蛋白 (GFAP)的阳性反应细胞的分布。显微镜下观察测量视网膜厚度 ,计数神经节细胞、GFAP免疫反应阳性细胞数。与青年猫比较 ,老年猫视网膜总厚度以及外核层、外网状层、内核层和内网状层厚度均显著减小 ;神经节细胞层的细胞密度显著下降 ;GFAP免疫反应阳性细胞显著增加 ,GFAP阳性细胞阳性反应强 ,胞体明显膨胀 ,突起稠密粗大。推测在衰老过程中视网膜细胞有神经元丢失现象 ,可能是造成视觉功能衰退的重要原因之一 ;视网膜星形胶质细胞的功能增强可能会延缓衰老。更多还原

【Abstract】 The retinae of 4 old cats (12 years,3-3.5 kg) and 4 young cats (1-3 years,2-2.5 kg) were selected.After treated in 4% paraformaldehyde,we applied Hematoxylin-Eosin and Nissl staining to exhibit the structure of the retinae and ganglion cells respectively,and also we used immunohistochemical ABC method to display the distribution of GFAP-immunoreactive (GFAP-IR) cells that were specifically classified as the astrocytes.By microscope we observed the thickness of retinae,counted the numbers of ganglion cells and GFAP-IR cells.Compared with young cats,the retinae in old cats presented a significant decrease in the thickness of total retina,outer nuclear layer,outer plexiform layer,inner nuclear layer,inner plexiform layer as well as an evidently lower density of ganglion cells.Also old cats showed clearly larger number and stronger staining of GFAP-IR cells than did in young cats,and at the same time the GFAP-IR cell bodies and dendrites swelled obviously in old cats.We presume that neurons lose in old cats,which hints that the loss of retinal cells might be of an important factor mediating visual functional declines during senescence.The functional increase of astrocytes may suspend more aging.更多还原