【摘要】 水生昆虫幼虫的种类鉴定是影响水质生物监测准确性的重要因素 ,我国这方面的形态学鉴定资料极其缺乏。本研究测定和分析了 4种鳞石蛾成虫及其疑似幼虫的mtDNA COⅠ、Ⅱ及tRNA基因序列 ,发现鳞石蛾成虫与其疑似幼虫的序列歧异度均 <1% ,属于种内差异 ,由此确认了 4种供试幼虫与其对应成虫的联系。研究表明利用线粒体DNA细胞色素氧化酶基因序列分析鉴定鳞石蛾类幼虫是可行的 ,为加快水生昆虫的幼虫分类研究提供了新途径。更多还原

【Abstract】 Identification of the larva stage of aquatic insects is one of the most important factors in biomonitoring water quality.So far,the morphological diagnostic character for many mature and early instar larvae are still lacking in China.The partial sequences of the mitochondrial COⅠ,COⅡ and tRNA gene of the adults and larvae of 4 species from Lepidostomatidae were sequenced and compared in this study.The results indicate that intraspecific divergence is <1% between the adult and larva and the interspecific divergence is 14%-24%.The study proves that the mitochondrial DNA sequence analysis is a useful tool to associate the larval and adult stages of Trichoptera,in turn;it will be valuable for health assessment of stream ecosystems.更多还原