【摘要】 目的 :探讨慢性脑供血不足对脑细胞内 [Ca2 + ]i,脑组织丙二醛 (MDA)及超氧化物歧化酶 (SOD)含量的影响 .方法 :采用高分子右旋糖酐法建立小鼠高血黏度动物模型 ,以Fluo 3/AM为细胞内游离钙离子的荧光指示剂 ,用激光扫描共聚焦显微镜测定脑细胞内 [Ca2 + ]i 的变化 ;用硫代巴比妥酸法及黄嘌呤氧化酶发光法分别测定脑组织匀浆MDA ,SOD含量 .结果 :高血黏度小鼠脑细胞内 [Ca2 + ]i 明显升高 (P <0 .0 1 ) ,脑组织匀浆中MDA含量明显升高 (P <0 .0 5 ) ,SOD含量明显降低 (P <0 .0 5 ) .相关分析显示小鼠全血高、低切黏度与脑细胞内 [Ca2 + ]i 均呈显著正相关关系 (Spearman等级相关系数分别为 0 .76 9,0 .82 1 ,P <0 .0 1 ) ;全血低切黏度与脑组织MDA含量也呈显著正相关关系 (Pearson相关系数为 0 .72 0 ,P <0 .0 1 ) .结论 :高血黏度可导致脑细胞内钙超载及脑组织中自由基反应增强更多还原

【Abstract】 AIM: To investigate the effects of chronic cerebral blood flow shortage caused by blood hyperviscosity on cytosolic free calcium concentration in cerebral cells, MDA and SOD contents in brain tissue. METHODS: Animal model of blood hyperviscosity was established by intravenous injection of high molecular dextran to mice. Alteration of [Ca 2+ ] i in murine cerebral cells was measured by confocal laser scanning microscope using Fluo 3/AM as calcium indicator, and MDA and SOD contents in brain tissue were determined. RESULTS: [Ca 2+ ] i in cerebral cells subjected to blood hyperviscosity increased significantly ( P <0.01) and [Ca 2+ ] i was positively correlated with the level of blood viscosity ( r η H=0.769, r η L=0.821, P <0.01). Compared with those of control, MDA content in the brain tissue rose but the SOD content dropped significantly ( P <0.05). The MDA content in the brain tissue was also positively correlated with the level of blood viscosity ( r η L=0.720, P <0.01). CONCLUSION: Blood hyperviscosity leads to Ca 2+ overload in cerebral cells and the increase of oxygen free radical reaction in brain tissues.更多还原