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水杨酸对水分胁迫黄瓜幼苗叶片生理过程的影响

Effects of salicylic acid on leaves of cucumber seedlings under water stress

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【作者】 黄清泉孙歆张年辉冯鸿袁澍代其林梁厚果杜林方林宏辉

【Author】 HUANG Qing-quan~1,SUN Xin~1,ZHANG Nian-hui~1,FENG Hong~(1,2),YUAN Shu~1,DAI Qi-lin~1,LIANG Hou-guo~1,DU Lin-fang~1,LIN Hong-hui~(1*)(1 College of Life Science,Sichuan University,Chengdu 610064,China;2 Sichuan Educational College,Chengdu 610041,China)

【机构】 四川大学生命科学学院四川大学生命科学学院 成都610064成都610064成都610064四川教育学院成都610041成都610064

【摘要】 研究了外源水杨酸 salicylic acid,SA 对水分胁迫下黄瓜幼苗叶片主要生理过程的影响 . 1m mol/L 的 SA处理黄瓜幼苗 2 4 h后 ,叶片中 POD活性剧增 ,SOD活性增加不明显 ,H2 O2 清除酶 CAT和 APX活性受抑制 ,H2 O2 含量上升引起膜脂过氧化产物 MDA含量上升 ,造成轻度氧化胁迫 ;在随后水分胁迫过程中 ,经 SA预处理积累的 H2 O2 诱导 APX和 CAT活性上升并清除产生的 H2 O2 ;SA预处理后 ,叶片中 Rubisco含量及其基因转录水平明显高于对照 ,光合作用受影响较小 .这表明 SA使黄瓜幼苗生理活性有较大改善 ,增强了植株对水分胁迫的抗性

【Abstract】 The effects of exogenous salicylic acid (SA) treatment on important physiological processes in leaves of cucumber seedlings under water stress were studied. After 24 hours treatment with 1 mmol/L SA, activity of POD increased dramatically. Activity of SOD didn’t increase conspicuously. Activities of CAT and APX, which are H2O2 removing enzymes, have been restricted. Increasing of H2O2 content results in the increasing of MDA (production of oxidation on membrane), and caused slight oxidation stress. In the subsequently progress of treatments, H2O2 accumulated after the pretreatment with SA made the activity of CAT and APX increase. H2O2 was eliminated then. Content and gene transcrip level of Rubisco in seedlings pretreated by SA was evidently higher than control. The influence of water stress on photosynthesis declined. This progress improved the physiological functions of the seedlings pretreated by SA in compared with the seedlings of control, and increased the endurance of cucumber seedlings in drought stress.

【关键词】 水杨酸黄瓜水分胁迫抗氧化Rubisco
【Key words】 salicylic acidcucumberwater-stressantioxidationRubisco
【基金】 国家自然科学基金 3 0 170 0 81;3 0 170 5 5 7 ;教育部博士点基金 ;四川省科技厅基金项目
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-occidentalia Sinica , 编辑部邮箱 ,2004年12期
  • 【分类号】S642.2
  • 【被引频次】97
  • 【下载频次】580
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