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藏药抱茎獐牙菜HPLC指纹图谱研究
Study on the chromatographic fingerprint of Tibet herb Swertia franchetiana by HPLC
【摘要】 采用反相高效液相色谱-二极管阵列的检测方法,对不同产地的10批野生和栽培抱茎獐牙菜药材的水溶性成分进行了分析,建立了抱茎獐牙菜药材的指纹图谱.色谱柱为VP-ODSC18柱 5μm,150mm×4.6mm ,流动相为甲醇-0.02%的磷酸水溶液,检测波长254nm.用文中的最佳条件可较全面地反映抱茎獐牙菜的主要成分,为藏药抱茎獐牙菜的质量控制提供了科学依据.
【Abstract】 The chromatographic fingerprint of Swertia franchetiana H.Smith has been set up by high performance liquid chromatography-diode-array detection method for ten batches herbs collected in different districts of Qinghai-Tibet Plateau.The analysis was performed on a VP-ODS C18 (5 μm,150 mm×4.6 mm) eluted with methanol and 0.02% aqueous phosphoric acid as mobile phase and with UV detection at 254 nm.A general fingerprint acquaintance of main constituents in S.franchetiana can be obtained by HPLC method,which provides scientific indexes for quality of S.franchetiana.
【Key words】 Tibet herb; Swertia franchetiana H.Smith; chromatographic fingerprint; high performance liquid chromatography-diode-array detection;
- 【文献出处】 西北植物学报 ,Acta Botanica Boreali-occidentalia Sinica , 编辑部邮箱 ,2004年11期
- 【分类号】R29
- 【被引频次】24
- 【下载频次】243