【摘要】 硫转运蛋白在植物对硫酸盐的吸收和转运中起着重要的作用。已经在拟南芥、大麦和小麦等植物中分离到了40多种硫转运蛋白基因。这些基因序列与其他种类生物的硫转运蛋白基因序列有着高度的保守性。利用CLUSTAL程序建立的系统进化树将植物硫转运蛋白划分为5个亚群。使用多种拓扑预测程序推测出不同植物硫转运蛋白的共同结构特点是均含有12个跨膜域。在柱花草和大麦中,硫转运蛋白基因表达调控包括植物体内硫水平的负调控和O-乙酰丝氨酸的正调控两种方式。对硫转运蛋白的组织定位和功能研究表明,高亲和硫转运蛋白主要定位于根部,在根系硫酸盐吸收中起重要作用。更多还原

【Abstract】 The plant sulphate transporters may play a key role on sulphate transport and sulphate absorption of plants. Over 40 genes of sulphate transporters have been cloned from Arabidopsis , wheat, barley, rice and potato, etc. Compare the gene sequences of sulphate transporters from plant to non-plant organisms, there have a high conservation. Using the program of CLUSTAL, phylogenetic analysis of plant sulphate transporter was been produced. The plant sulphate transporters were been divided into 5 subtypes. To apply various topology prediction programs to analysis the amino acid sequences of sulphate transporters, the results have been shown that sulphate transporters have 12 membrane spanning domains (MSD). In the Stylosanthes hamata and barley, there have two regulation patterns: a negative feedback by sulphur level in plant and a positive feedback by O-acetylserine. The sulphate transporters, which have a high affinity for sulphate, were expressed in root tissues and have a principally responsible for primary uptake of sulphate.更多还原