【摘要】 研究了蛋白核小球藻在增强UV-B辐射下NO信号的产生.结果表明:NO释放量与UV-B的强度相关.在藻类细胞培养液中加入NOS的竞争性抑制剂硝基精氨酸 LNNA ,不能抑制NO的释放.加入NO清除剂乙酰半胱氨酸 NAC ,能明显减少NO的释放.在无氮和有氮培养基中NO同样的释放.这些结果说明NO生成途径不经过依赖L-精氨酸的NOS和依赖NO-3和硝酸还原酶,NO的底物不是L-精氨酸和NO-3.在无氮和有氮培养基中同样有NO-2的形成,NAC减少NO-2的释放,说明生成了NO-2有可能是NO释放后生成的.更多还原

【Abstract】 It was investigated the effect of UV-B radiation （280～320 nm） on induction of nitric oxide （NO） in the green alga Chlorella pyrenoidosa suspensions. N^w-nitro-Larginine （LNNA）, an inhibitor of NO synthase （NOS）, had not any inhibitory effect on the rate of NO production. N-Acetyl-L-cysteine （NAC）, a scavenger of NO, blocked NO generation. Cultured in nutrient solution with or without nitrate （NO^-₃, nitrate-starved 24hours） suspensions, the cells of Chlorella pyrenoidosa similarly generated NO and NO^-₂. The results indicate that NO^-₃ was not substrate of NO and NO^-₂ and also indicate that NO induced by UV-B radiance not through L-Arg-dependent NOS and NO^-₃. NO^-₂ that released in the alga suspensions were measured. NO^-₂ could be generated from NO.更多还原