【摘要】 聚合酶链式反应 -单链构像多态性分析 (PCR-SSCP)是基因突变分析中的一项重要技术。是现代遗传学研究中用于己知基因突变检测和未知基因突变筛查的一种非常有用的工具。本文简要介绍了 PCR-SSCP的原理和方法、发展的历史、影响分析结果的因素 ,分析了该技术的优缺点和应用前景 ,特别阐述了在检测氡暴露早期损伤方面的价值更多还原

【Abstract】 Single strand conformation polymorphism analysis of polymerase chain reaction products, PCR-SSCP, is an important aspect of gene mutation analysis and also a highly useful tool in identifying unknown gene mutation. The principles, methods and development history of the PCR-SSCP analytical technique were briefly described. The factors affecting analytical results, as well as the advantages and disadvantages of the technique, were analyzed in this paper, including its future applications, such as a potential value in measuring the early damage from the exposure of radon.更多还原