【摘要】 目的　探讨干扰素的体外抗乙型肝炎病毒作用。方法　以HepG2 2 ,2 ,15为细胞模型 ,实验共观察 9d ,于第 3、6、9d收集培养上清行HBsAg定量检测 ;收集细胞提取总RNA ,行RT -PCR及荧光实时定量PCR测细胞内乙肝病毒mRNA水平。结果　实验第 3d及第 6d ,对照组和各实验组之间HBsAg及病毒mRNA水平无显著性差异 ;实验第 9d ,各实验组HBsAg及病毒mRNA水平均较对照组明显降低 ,α干扰素浓度为 5 0 0IU/ml抑制作用达最大。结论　干扰素在实验浓度下可抑制HepG2 2 ,2 ,15细胞内乙肝病毒基因的转录和表达 ,具有直接抑制乙肝病毒复制作用更多还原

【Abstract】 Objective To evaluate the in vitro inhibiting activities of interferon-alpha (INF-α) against hepatitis B virus (HBV). Methods HepG2 2 2 15 cells were treated with INF-α. At 3rd, 6th and 9th days after treatment, the supernatant was collected for HBsAg quantitative assay, and total RNA of the cells was isolated for RT-PCR and realtime-PCR assay of HBV mRNA. Results There were no significant differences in HBsAg titer and virus mRNA level at 3rd and 6th days between the experimental and control groups. At 9th day, HBsAg titer and virus mRNA level in INF-α group were significantly lower than those in control group. And 500IU/ml INF-α could get maximal inhibiting effect against HBV. Conclusion INF-α can inhibit transcription and expression of HBV gene in HepG2 2 2 15 cells, which indicated that INF-α can inhibit HBV replication directly.更多还原