【摘要】 目的　研究食管鳞癌中TGF -β1 、CDK4的表达。方法　用免疫组织化学方法 (SABC法 )研究TGF -β1 和CDK4蛋白表达 ,显微镜下计数阳性细胞指数 (LI) ,并进行统计分析。结果　 1、TGF -β1 的表达 :正常食管黏膜阳性率达 2 0 %( 2 /10 ) ,食管鳞癌组织 62 %( 3 1/5 0 )两者差异有显著性 (P =0 0 5 ) ;LI分别为 ( 5 5 88± 6 186)及 ( 2 9 469± 2 7 481) ,后者高于前者 (P =0 0 1) ,正常食管黏膜弱阳性 2例。 2 .CDK4表达 :正常食管黏膜组织阳性率为 10 %( 1/10 ) ,食管鳞癌组织为 5 0 %( 2 5 /5 0 ) ,两者比较差异有显著性 (P<0 0 5 ) ,LI分别为 ( 3 15 8± 4 40 0 )、( 2 4 0 11± 2 6 965 ) ,(P =0 0 5 ) 1例正常食管黏膜阳性者为弱阳性 (LI <2 5 %)。结论　食管鳞癌中存在TGF -β1 的高表达 ,与正常粘膜差别显著 ,TGF -β1 的检测有辅助诊断作用 ;CDK4在食管鳞癌中高表达 ,促进了细胞生长和肿瘤发展 (G1 →S过渡 ) ,反映了肿瘤的生物学特性 ,是食管癌发生发展中的重要事件。更多还原

【Abstract】 Objective To study the roles of TGF-β 1 and CDK4 in esophageal squamous cell carcinoma (SCC).Methods The expression of TGF-β 1 and CDK4 was examined in 50 cases of esophageal squamous cell carcinoma and 10 cases of normal esophageal mucosa with SABC immunohistochemical technique. All the samples were stained by HE to confirm their pathologic characters. The number of positive cells was counted under microscope. The data were analyzed with x 2 tests and t test.Results TGF-β 1 and CDK4 positive cells were significantly higher in esophageal SCC than those in the normal esophageal mucosa (P<0.05).Conclusions TGF-β 1 is overexpressed in esophageal SCC. Determination of TGF-β may be helpful to diagnose squamous cell carcinoma.CDK4 overexpression in esophageal SCC may promote the cells growth and cancer development(GS transition), and it reflects tumor biological characteristics, and is a major event of tumor development.更多还原