节点文献
转γ-生育酚甲基转移酶基因大豆的获得
TRANSFORMATION OF SOYBEAN WITH γ-TMT FROM BRASSICA OLERAEA FOR α-TOCOPHEROL ELEVATION
【摘要】 以大豆子叶节为外植体 ,利用γ -生育酚甲基转移酶基因 (γ -TMT )的种子特异性表达载体 p7S -TMTL ,通过农杆菌介导法转化大豆 ,获得 2 6株抗卡那霉素再生植株。PCR检测结果表明其中 4株为阳性 ,初步证明γ -TMT已整合到这 4株大豆的基因组中。
【Abstract】 Transformation of soybean with seed specific expression vector p7S-TMTL was conducted through Agrobacterium-mediated co-transformation. Twenty-six Kanamycin resistant plants were examined by PCR with specific primers. Four plants showed positive signals, indicating that γ-TMT was transformed into the genome of these four plants.
【关键词】 大豆;
γ-TMT;
α-生育酚;
农杆菌介导;
子叶节;
PCR;
【Key words】 Soybean; γ-TMT; α- tocopherol; Agrobacterium-mediated co-transformation; Cotyledonary node; PCR;
【Key words】 Soybean; γ-TMT; α- tocopherol; Agrobacterium-mediated co-transformation; Cotyledonary node; PCR;
【基金】 国家自然科学基金 (批准号 :3 0 2 70 85 6);国家重点基础研究发展计划 ( 2 0 0 2CB1113 0 1)资助
- 【文献出处】 大豆科学 ,Soybean Science , 编辑部邮箱 ,2004年03期
- 【分类号】S565.1
- 【被引频次】5
- 【下载频次】109