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家蚕与中国野桑蚕的RAPD标记遗传多样性比较分析

Comparative Analysis of Genetic Polymorphism of Silkworm(Bombyx mori) and Wild Mulberry Silkworm (Bombyx mandarina)

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【作者】 刘玲玲潘敏慧代方银余红仕鲁成

【Author】 LIU Lingling PAN Minhui DAI Fangyin YU Hongshi LU Cheng (The Key Sericultural Laboratory of Agricultural Ministry,College of Sericulture and Biotechnology of Southwest Agricultural University,Chongqing 400716)

【机构】 西南农业大学蚕学与生物技术学院农业部蚕桑学重点开放实验室农业部蚕桑学重点开放实验室 重庆4100716重庆4100716重庆4100716

【摘要】 对家蚕品种C10 8及江苏地区野桑蚕进行了RAPD分析 ,并利用已有的不同家蚕品种及其它地区野桑蚕的RAPD数据 ,比较了家蚕品种内和野桑蚕地理种群内个体间、家蚕品种间和野桑蚕地理种群间的遗传多样性。家蚕品种内的多态位点比率、Shannnon信息指数、Nei基因多样性指数等都远较野桑蚕地理种群内个体间的小 ,2 5个家蚕品种在分组和未分组时的遗传多样性指数仅有一组例外 ,其余均较野桑蚕地理种群间的大。由此说明家蚕品种内的遗传多样性比野桑蚕地理种群内个体间的低 ;而家蚕品种间的遗传多样性水平却比野桑蚕地理种群间的高。未分组家蚕品种间的遗传多样性指数略大于分组后的 ,表明分析比较的样本数不同对结果也有一定影响。

【Abstract】 We analysized the genetic polymorphism of one strain of silkworm ( B.mori ) and one geographic wild mulberry silkworm ( B.mandarina ) by random amplified polymorphism DNA (RAPD).Furthermore,combined with the primary data,the genetic polymorphism of intrastrains of silkworm( B.mori ) and geographic intrapopulations of wild mulberry silkworm ( B.mandarina ) and that of interstrains of silkworm ( B.mori ) and geographic interpopulations of wild mulberry silkworm ( B.mandarina ) have been compared.The result showed that the percentage of polymorphic loci,Shannnon’s information index and Nei’s index of intrastrains of silkworm ( B.mori ) are lower remarkably than that of geographic intrapopulation of wild mulberry silkworm ( B.mandarina ),while grouped or ungrouped interstains of silkworm ( B.mori ) are higher than geographic interpopulations of wild mulberry silkworm ( B.mandarina )(one group exception).It suggested that genetic polymorphism of intrastrains of silkworm ( B.mori ) is lower than that of geographic intrapopulations of wild mulberry silkworm’s ( B.mandarina ),and that of interstains of silkworm’s ( B.mori ) is higher than geographic interpopulations of wild mulberry silkworm’s ( B.mandarina ).In addition,all genetic polymorphism index of ungrouped strains are higher slightly than that of grouped strains,which indicated that different sample size may have a effect on results.

【基金】 国家自然科学基金 (编号 3 0 170 719) ;教育部重点项目 (编号 0 2 40 7)。
  • 【文献出处】 蚕业科学 ,Acta Sericologica Sinica , 编辑部邮箱 ,2004年03期
  • 【分类号】S881
  • 【被引频次】9
  • 【下载频次】89
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