【摘要】 为了克隆Drosomycin基因用于今后定点诱变修饰和生物技术开发研究 ,根据果蝇Drosomycin基因 (drs)的cDNA序列 ,分别设计Drosomycin基因含和不含信号肽序列的两对特异引物Drs1/Drs2、Dro3/Dro4 ,由黑腹果蝇(DrosophilamelanogasterMeigen)基因组PCR扩增获得目的片段drs和dro ,克隆到表达载体pET 2 1d(+)和pHIL S1之中。经测序表明 ,克隆的drs与文献报道的drs序列仅在 +116位点有 1个碱基的差异 (A/T) ,而克隆的dro序列则与文献报道的dro序列完全相同。更多还原

【Abstract】 To clone Drosomycine gene for modification of point mutagenesis and biotechnique utilization, two pairs of the special primers, Drs1/Drs2 and Dro3/Dro4, with or without signal peptide sequence, respectively, were designed according to cDNA sequence of Drosomycine gene (drs) to obtain target drs and dro fragments by PCR from the genome of Drosophila melanogaster Meigen.The amplified fragments of drs and dro were cloned into expression vector pET-21d(+)and pHIL-S1, respectively. The results of the sequencing drs and dro fragments inserted two vectors showed that there is only one base difference (+116 locus: A/T) between the cloned drs sequence and the drs sequence in the references, the cloned dro sequence is quite same as the dro sequence in the references.更多还原