【摘要】 目的 探讨骨髓基质细胞向神经元方向分化后是否具备神经元的电生理功能。方法 应用膜片钳技术,釆用全细胞记录方式.对由星形胶质细胞诱导的分化前、分化3d、分化7d的成年大鼠骨髓基质细胞进行电生理功能测定。结果分化前后的骨髓基质细胞未记录到内向Na~+电流,但成功记录到了静息膜电位,分別为:(-12.71±3.25)mV(n=7)、(-26.29±10.34)mV(n=7)、(-45.0±8.46)mV(n=6);刺激脉冲为40 mV时三组的外向延迟整流K~+电流分別为:(0.31±0.17)nA(n=7)、(1.34±0.59)nA(n=8)、(1.99±0.97)nA(n=7);刺激脉冲为-40 mV时的内向整流K~+电流为:(79±58)pA(n=7)、(260±150)pA(n=8)、(420±120)pA(n=7),伴随着分化时间的逐渐延长,两种K~+电流及膜电位逐渐增高。结论(1)骨髓基质细胞表达弱的外向延迟整流K~+电流及内向整流K~+电流可能是其特征性的电生理指标。(2)骨髓基质细胞经过星形胶质细胞的诱导能够向功能性神经元方向分化。更多还原

【Abstract】 Objective To study the electrophysiological functions of neurons derived from adult rat marrow stromal cells (rMSCs). Methods Adult rMSCs were cocultured with astrocytes isolated from the hippocampus of newborn rat,and then were divided into three groups:before,3 d and 7 d after differentiation. The membrane currents and resting membrane potentials were recorded by using whole cell patch clamp technique. Results 3 groups of cells expressed both outward and inward K~+ currents with no evidence for Na~+ currents. The density of the delayed outwardly rectifying K~+ currents at 40m Vwere (0.31±0.17) nA (n=7), (1.34±0.59) nA (n=8) and (1.99±0.97) nA (n=7) respectively, and that of the inwardly rectifying K~+ currents at -40mV were (79±58) pA (n=7), (260±150) pA (n=8) and (420±120) pA (n=7) respectively. The resting membrane potentials were (-12.71±3.25) mV (n=7), (-26.29±10.34) mV (n=7) and (-45.0±8.46) mV (n=6) respectively. Conclusion (1)The absence of Na~+ currents combined with the presence of weakly voltage-activated K~+ currents might be the electrophysiotogical characteristic features of rMSCs. (2)MSCs can differentiate into functional neurons if induced by astrocytes.更多还原