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三种黄鳝RAPD分析的引物筛选

The Selection of Polymorphic Primers to Three Species of Monopterus by RAPD Analysis

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【作者】 张继平贺顺连周孟娇郭金彪张绮琼

【Author】 ZHANG Ji-ping~1, HE Shun-lian~1,ZHOU Meng-jiao~2, GUO Jin-biao~1, ZHANG Qi-qiong~1 (1.Department of Animal Science, Foshan Science and Technology College, Foshan 528231,China; 2.College of Animal Science,Hunan Agriculture University, Changsha 410128,China)

【机构】 广东佛山科学技术学院动物科学系湖南农业大学动物科技学院广东佛山科学技术学院动物科学系 广东 佛山 528231广东 佛山 528231湖南 长沙 410128广东 佛山 528231

【摘要】 试验用随机扩增多态DNA(RAPD)技术对黄鳝属中产自缅甸的山黄鳝、印度尼西亚的穴黄鳝及中国的黄鳝的混合DNA进行引物筛选。40条引物有35条引物扩增出多态性片段。35条引物共获得334个扩增片段,长度为250~5417bp。

【Abstract】 The samples of three species of synbranchi form eels,Monopterus cuchia,M.fossorius and M.albus were collected from Yangon of Myanmar,Indonesia and Yuanjiang County, Hunan Province,China respectively.Randomly amplified polymorphic DNA (RAPD) technique was used to analyse the selection of polymorphic primers of the wild grouper population of four individuals from each species.35 out of 40 polymorphic priners amplified diversity.35 primers produced 334 fingerprints with the molecular length varying from 250 bp to 5417 bp.

  • 【分类号】S917
  • 【被引频次】1
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