【摘要】 目的 :通过对胶质瘤的促血管生成素 1 (Angiopoietin 1 ,Ang 1 )和促血管生成素 2 (Angiopoietin 2 ,Ang 2 )及酪氨酸激酶受体 (Tie 2 )mRNA表达的检测 ,初步探讨它们与胶质瘤血管生成的关系及临床意义。方法 :应用RT PCR对 4 3例胶质瘤和 1 5例正常脑组织Ang 1、Ang 2及Tie 2mRNA的表达量进行半定量检测 ,并对它们进行对照分析。结果 :胶质瘤组织中Ang 2mRNA的表达明显增强 ;Ang 2mRNA的表达随着肿瘤级别的升高而增强 (P <0 0 0 1 ) ;胶质瘤组织中Ang 1mRNA的表达较正常脑组织下调 ,Ⅲ、Ⅳ级胶质瘤Ang 1mRNA的表达量与Ⅰ、Ⅱ级相比 ,其降低程度也有着显著性差异 (P <0 0 5 ) ;Tie 2mRNA的表达量在正常脑组织与胶质瘤、以及不同分级之间无显著性差异。结论 :Ang 2mRNA表达上调和Ang 1mR NA表达下调可能共同诱发肿瘤血管生成。Ang 2与Ang 1平衡状态失衡可能与胶质瘤恶性程度有关更多还原

【Abstract】 Objective:To determine the expression of angiopoietin 1(Ang 1),angiopoietin 2(Ang 2) and tyrosine kinase receptor Tie 2 in glioma and assess the role of angiogenesis.Methods:Forty three patients with glioma and 15 patients with normal brain tissues were included in this study.The expression of Ang 1,Ang 2 and Tie 2 mRNA was analyzed by semiquantitative RT PCR.Results:Our results showed increased expression of Ang 2 in glioma.Furthermore,we found a strong association between expression of Ang 2 and tumor progression.On the other hand,decrease of Ang 1 was found in glioma,and Ang 1 level in grades Ⅲand Ⅳ was significantly lower than that in grades Ⅰ and Ⅱ.There was no obvious difference between Tie 2 levels in glioma and normal brain tissue.Conclusion:Enhanced expression of Ang 2 and down regulation of Ang 1 in glioma are all involved in tumor angiogenesis.The imbalance between Ang 1 and Ang 2 may be a critical factor which influences biologic behavior of glioma.更多还原