【摘要】 目的 :研究从人胚胎关节软骨中分离的细胞的特性、对其表面标志进行鉴定 ,探讨其多向分化潜能。方法 :从 12～ 2 0周人胚胎关节软骨中分离细胞 ,观察细胞的形态、生长状态 ,采用流式分析的方法对其表面标志进行鉴定并探讨其成骨、成脂、成软骨以及成神经元样细胞的多向分化的潜能。结果 :(1)从人胚胎关节软骨中分离的细胞 ,细胞形态均一 ,以梭形为主 ,生长旺盛 ,可传 2 0代。 (2 )对不同代的细胞进行流式鉴定 ,发现其表面标志CD4 4、CD 14 7、CD 90为阳性 ,CD 34、CD 4 5、HLA DR为阴性 ,与骨髓来源的间充质干细胞的表面标志相似。 (3)对其进行成骨、成脂、成软骨、成神经元样细胞的诱导 ,发现软骨来源的细胞具有多向分化的能力。结论 :从人胚胎关节软骨中可获得间充质干细胞。更多还原

【Abstract】 Objective: To verify the hypothesis that cells with characteristics similar to bone marrow mesenchymal stem cells(MSCs) can be isolated and cultured from human fetal articular cartilage. Methods: Human fetal articular cartilages were harvested from fetuses aborted between 12 and 20 weeks. Cells were grown in monolayer cultures in IMDM medium containing antibiotics, L-glutamine and fetal calf serum. Cells were induced to differentiate into adipocytes, osteoblasts, chondrocytes, and neurons. At various time points, parental and passaged cells were subjected to FACS analysis to determine cell phenotype. Results: We successfully isolated and cultured MSCs from human fetal articular cartilage. These cells had the same morphology, phenotype, and ability to differentiate in vitro as MSCs of bone marrow origin. Conclusion: This study shows that cells with characteristics of MSCs can be isolated and cultured from human fetal articular cartilage.更多还原