【摘要】 目的 :观察不同类型维生素E(α、γ、mixed tocopherols)对氧化型低密度脂蛋白 (oxLDL)及重组人C反应蛋白 (rhCRP)诱导的人脐静脉内皮细胞 (HUVECs)细胞间黏附分子 1 (ICAM 1 )表达的影响 ,以探讨不同类型维生素E的抗炎、抗动脉粥样硬化机制及不同效果。方法 :体外培养HUVECs,分别加oxLDL、oxLDL +α tocopherol、oxLDL +γ tocopherol、oxLDL +mixed tocopherols、rhCRP、rhCRP +α tocopherol、rhCRP +γ tocopherol、rhCRP +mixed tocopherols孵育 2 4h ,采用细胞酶联免疫吸附 (ELISA)、流式细胞、RT -PCR技术分别测定ICAM 1蛋白和mRNA表达水平。结果 :oxLDL和rhCRP均可诱导HUVECs的ICAM 1蛋白和mRNA表达增加 ,不同类型维生素E均可抑制oxLDL诱导的HUVECs的ICAM 1蛋白和mRNA表达 ,并呈浓度依赖性 (5 0～ 2 0 0 μmol/L)。与相同浓度的α、γ tocopherol相比 ,mixed tocopherols抑制作用最强。不同类型维生素E均不能抑制rhCRP诱导的HUVECs的ICAM 1蛋白和mRNA表达。结论 :不同类型维生素E均可抑制oxLDL诱导的HUVECs的ICAM 1蛋白和mRNA表达 ,与α、γ tocopherol相比 ,mixed tocopherols对oxLDL诱导HUVECs表达ICAM 1的抑制作用最强更多还原

【Abstract】 Objective: To observe the influence of different tocopherol isoforms on oxidized low density lipoprotein (oxLDL) or recombinant human C reactive protein (rhCRP) induced expression of intercellular adhesion molecule 1 (ICAM 1) in human umbilical vein endothelial cells (HUVECs) and to investigate the potential mechanisms and effects of different tocopherols on atherosclerosis. Methods: Cultured HUVECs were incubated with oxLDL,oxLDL+α tocopherol,oxLDL+γ tocopherol,oxLDL+mixed tocopherols,rhCRP,rhCRP+α tocopherol,rhCRP+γ tocopherol, rhCRP+mixed tocopherols for 24 hours, respectively. The ICAM 1 expressions of protein and mRNA were detected by cell enzyme linked immunosorbent assay (ELISA), flow cytometric technique and RT-PCR. Results : Incubation of HUVECs with oxLDL or rhCRP for 24 hours significantly increased ICAM 1 expressions of proteins and mRNA . The different tocopherols inhibited oxLDL induced ICAM 1 expression in HUVECs in a concentration dependent manner(50-200 μmol/L) and mixed tocopherols were more potent than α tocopherol or γ tocopherol alone. However, rhCRP induced ICAM 1 expression in HUVECs was not inhibited by tocopherols. Conclusion :The different tocopherols inhibited oxLDL induced ICAM 1 expression in HUVECs and mixed tocopherols were more potent than α tocopherol or γ tocopherol alone, which may be important for the beneficial effects of tocopherols on atherosclerosis and cardiovascular disease.更多还原