【摘要】 目的 :在体外条件下检测重组人透明带蛋白诱导的顶体反应。方法 :体外采集已生育健康男子精液 ,采用非连续密度梯度法分离精子 ,将获能的精子悬液分成阴性对照组 (C组 ,2 0 0 μL 精子悬液加 1μL DMSO)、阳性对照组 (A组 ,2 0 0 μL精子悬液加 A2 3187至终浓度为 10 μmol· L- 1 )和 ZP3组 (2 0 0 μL 精子悬液加 ZP3至终浓度为 10 m g· L- 1 ) ,考马斯亮蓝染色法检测重组人 ZP3蛋白诱导的顶体反应。结果 :C组、A组和 ZP3组发生顶体反应的精子百分率分别为 (5 .0 0± 1.70 ) %、 (4 6 .10± 7.4 9) %和 (13.2 0± 2 .70 ) % ,各组间比较差异有显著性 (P<0 .0 5 )。结论 :重组人透明带蛋白能够诱导人精子发生顶体反应。更多还原

【Abstract】 Objective To detect the acrosome reaction induced by recombinant human zona pellucida 3 (rhZP3) in vitro. Methods The sperm from fertilized healthy men were obtained in vitro. The spermatozoa were isolated by discontinuous density gradient centrifugation, and the capacitated spermatozoa suspension were divided into negative control group (group C, 200 μL spermatoza suspension plus 1 μL DMSO), positive control group (group A, 200 μL spermatozoa plus A23187 to the end concentration of 10 μmol·L -1) and ZP3 group (200 μL spermatozoa plus ZP3 to the end concentration of 10 mg·L -1). The acrosome reaction induced by rhZP3 was detected with coomassie brilliant blue staining. Results The percentages of acrosome reaction of spermatozoa in group C, group A, and ZP3 group were (5.00±1.70)%,(46.10±7.49)%, and (13.20±2.70)%,respectively. There were significant differences between various groups (P<0.05).Conclusion The rhZP3 can induce acrosome reaction of spermatozoa.更多还原