【摘要】 目的 :探讨脐带血和脐带血管肾上腺髓质素 (ADM)和内皮素 1 (ET- 1 )在妊娠高血压综合征 (简称妊高征 )中的作用。方法 :应用放射免疫法测定 30例妊高征患者和 1 2例正常妊娠晚期孕妇 (简称正常晚孕组 )脐静脉血浆 ADM和 ET- 1水平 ,用免疫组化法检测 ADM和 ET- 1蛋白在 4 0例妊高征患者和 1 2例正常妊娠晚期孕妇脐带血管中的表达。结果 :1妊高征患者脐静脉血浆 ADM水平高于正常晚孕妇女 ,且随着病情加重逐渐增高 ,中、重度妊高征患者与正常晚孕组比较差异也具有显著性 (P<0 .0 5 )。妊高征患者脐静脉血浆 ET- 1水平略有升高 ,与正常晚孕组比较 ,差异无显著性 (P>0 .0 5 ) ;2正常晚孕组脐带血管内皮细胞和平滑肌细胞有 ADM的表达 ,妊高征组随着病情加重 ,ADM表达增强 ;正常晚孕妇女 ET- 1在脐带血管的内皮细胞表达 ,妊高征组随着病情加重 ,ET- 1表达减弱。结论 :脐血和脐带血管 ADM和 ET- 1表达改变可能与妊高征胎儿胎盘之间血液循环调控有关更多还原

【Abstract】 Objective To investigate the role of adrenomedullin (ADM) and endothelin 1 (ET 1) in the pathogenesis of pregnancy induced hypertension (PIH). Methods The plasma concentrations of ADM in human umbilical vein of PIH patients( n =30) and normal late trimester pregnancy women ( n =12)were measured by radioimmunoassay. The expressions of ADM and ET 1 in umbilical cord vessel of PIH patients( n =40)and normal late trimester pregnancy women ( n =12) were detected by immunohistochemistry (SABC) . Results ①The plasma concentration of ADM in human umbilical vein of PIH patients was significantly higher than that of normal late trimester pregnancy women (P<0 05). The plasma concentration of ET 1 in human umbilical vein of PIH patients was similar to that of normal late trimester pregnancy women ( P >0 05). ②The expression of ADM was found in endothelium and smooth muscle cell of umbilical cord vessel,and it increased with the serious degree of PIH. The expression of ET 1 was only found in endothelium of umbilical cord vessel, and it decreased with the serious degree of PIH. Conclusion The changes of ADM and ET 1 in umbilical cord plasma and vessel may related to regulation of fetoplacental circulation in PIH.更多还原