【摘要】 该文以MS培养基为基本培养基 ,两个品种的高羊茅种子为外植体 ,接种在附加 2 ,4 D、KT、6 BA、NAA、IAA、水解乳蛋白等不同激素和营养物质的培养基上 ,研究了诱导高羊茅种子愈伤组织形成的主要因素 .结果发现在不同种类和浓度的激素条件下 ,愈伤组织的诱导率有很大的差异 ,2 ,4 D是诱导高羊茅种子形成愈伤组织的关键因素 ,而KT、6 BA、NAA、IAA则没有明显的效果 ,并从多种培养基组合中筛选出了诱导愈伤组织的最佳培养基 ,高羊茅种子的愈伤组织诱导以MS + 6mg L 2 ,4 D + 50 0mg L水解乳蛋白为最佳 .通过愈伤组织的继代和分化培养 ,诱导出丛生芽 ,分化培养基以MS + 4mg L 2 ,4 D + 0 .2mg LKT为最佳 ,初步建立起高羊茅的植株再生体系更多还原

【Abstract】 In this paper, two species of tall fescue seed were cultivated on the MS medium supplemented with different kinds of hormones and nutrients. The effect of 2,4--D,KT, 6--BA, NAA,IAA,lactalbumin hadroly satz and culture c onditions for inducing callus from Festuca arundinacea seeds were described . The frequency of callus induction was different when the same kind of Festuca arundinacea seeds was cultivated in MS medium supplemented w ith different types of and different concentrations of hormones. It was shown that 2,4--D decided the frequency of callus induced from t all fescue seeds, while KT, 6-- BA,NAA,IAA had no significant effect on the callus. The optimum callus in ducing medium of Festuca arundinacea seeds was selected from these kinds of media,and the best one was MS + 6 mg/L 2,4--D + 600 mg/L l actalbumin hadrolysatz. The authors successfully regenerate tall fescue plant by means of cultivating tall fes cue callus on the MS medium supplemented with 2,4--D and KT,a nd th e best plant regeneration medium of tall fescue was MS + 6 mg/L 2,4--D +0.2 mg/L KT. A preliminary plant regeneration system of Festuca ar undinacea was established on the MS medium supplemented with appropriate hormones and nutrients by means of inducing callus and regenerating plants.更多还原