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秋水仙碱诱导白杨三倍体新途径的发现

Discovery of a new way of poplar triploids induced with colchicine after pollination.

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【作者】 康向阳张平冬高鹏赵芳

【Author】 Kang Xiangyang;Zhang Pingdong;Gao Peng;Zhao Fang. MOE Key Lab. for Genetics & Breeding of Forest Trees and Ornamental Plants, Beijing For. Univ., 100083, P.R.China.

【机构】 北京林业大学林木花卉遗传育种教育部重点开放实验室北京林业大学林木花卉遗传育种教育部重点开放实验室

【摘要】 以银腺杨等为母本 ,毛白杨为父本 ,授粉后一定时间采用秋水仙碱溶液处理花序 ,共获得 40 2粒种子 ,成苗10 7株 ,从中检出 2 1株银腺杨×毛白杨三倍体 .证明授粉后 2 4~ 3 6h是施加秋水仙碱诱导白杨三倍体的有效时期 ,三倍体得率最高达 57 1% .通过这一途径获得的白杨三倍体同样具有十分明显的生长优势 ,2年生实生苗的平均苗高、地径超出全部后代群体总平均值的 2 9%和 65% ,其中以BY3 2 0等表现最佳 ,分别超出群体平均数的 55%和119% .该研究为高效诱导白杨三倍体找到了一条新的途径

【Abstract】 Populus alba×P. glandulosa)×P. tomentosa triploids were found from the 107 seedlings,which were obtained from 402 hybrids between P. alba×P. glandulosa etc. (male parent) and P. tomentosa(female parent) by treating the pollinated flowers with colchicine. It indicated that the effective period of inducing triploids with colchicine was 24~36 h after pollination. The highest ratio of obtained triploids reached 57 1%. The growth of triploids also showed obvious superiority over normal diploids. The average stem height and basal diameter of 2 year old triploid seedlings was 29% and 65% larger than the average growth of all the offspring respectively. The growth of BY320 is the best,of which stem height and basal diameter was 55% and 119% higher than the average growth respectively. A new way for inducing poplar triploid was obtained through the present research.

【关键词】 白杨授粉秋水仙碱三倍体
【Key words】 poplarpollinationcolchicinetriploid
【基金】 国家林业局重点项目 ( 1 999 1 2 )的部分内容
  • 【文献出处】 北京林业大学学报 ,Journal of Beijing Forestry University , 编辑部邮箱 ,2004年01期
  • 【分类号】S792
  • 【被引频次】91
  • 【下载频次】504
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