【摘要】 运用反向PCR (IPCR)技术首次克隆得到全长为 3 50 6bp的中华绒螯蟹 (Eriocheirjaponicasinensis)蜕皮抑制激素 1(MIH 1)基因组DNA序列 (GenBank检索号 :AY3 10 3 13 )。该序列包括 3个外显子、 2个内含子、 412bp的 5′端上游调控区和 917bp的 3′端UTR。编码区的第 1个内含子将信号肽分开 ,第 2个内含子将成熟肽分开。MIH 1基因的外显子和内含子接头区符合受体拼接点和供体拼接点的GT AG法则。MIH 1基因412bp的 5′端侧翼区含有和其它真核基因相似的启动子元件 ,即包括与其它节肢动物高度相似的起始子、TATA盒以及cAMP效应元件结合蛋白的结合位点序列。中华绒螯蟹MIH 1基因的组织方式与斑纹和食用黄道蟹的MIH基因相同。推导的多肽由 75个氨基酸的成熟肽和 3 5个氨基酸的信号肽组成 ,成熟肽的氨基酸序列和食用黄道蟹、三叶真蟹及美洲黄道蟹的一致性在 64% -65%之间更多还原

【Abstract】 A full-length sequence of molt-inhibiting hormone 1 (MIH 1) genomic DN A from Eriocheir japonica sinensis (GenBank Accession No: AY310313) was clo ned by the inverse-PCR method. The sequence is 3 506 bp in size and consists of 3 e x ons, 2 introns, 412 bp of 5′ upstream regulatory region and 917 bp of 3′ UTR. Th e first intron separates the signal peptide and the second intron separates the mature peptide in the coding region. The exon-intron boundary of the Ers-MIH 1 g ene follows Chambon’s rule for the splice donor and acceptor sites. The 41 2 bp of the upstream 5′ flanking region of the MIH 1 gene contains promoter elements with characteristics similar to other eukaryotic genes. These included sequences with high degrees of similarity to the arthropod initiator, TATA box and cAMP r esponse element binding protein. The organization of the Ers-MIH 1 gene is iden t ical to that of the molt-inhibiting hormone gene of Charybdis feriatus and Cancer pagurus. The deduced polypeptide consists of a 75-amino acid mature peptide a nd a 35-amino acid region of signal peptide. The mature peptide shares amino a c id identity 64%-65% to the MIH from Cancer pagurus,Carcinus maenas and Ca ncer magister.更多还原