【摘要】 目的 :探讨实时荧光定量PCR(FQ PCR)检测乙型肝炎病毒DNA的临床应用价值。方法 :采用FQ PCR方法检测1 96例患者血清中HBV DNA。结果 :1 1 2例HBeAg(+) /HBeAb(- )标本中FQ PCR均阳性 ,HBV DNA拷贝数 1 .0 8× 1 0 7/ml;6 8例HBeAg(- ) /HBeAb(+)标本中 ,平均拷贝数 6 .1 2× 1 0 5/ml,其阳性率为 6 6 .2 % ;1 6例HBeAg(- ) /HBeAb(- )标本中 ,平均拷贝数为 2 .72× 1 0 4/ml。结论 :实时FQ PCR可以实现准确定量 ,是了解HBV在体内复制和判断疗效的有利手段更多还原

【Abstract】 Objective:To explore the role of the real time fluorescence quantitative polymerase chain reaction(FQ PCR) in detecting heptitis B virus(HBV) DNA.Methods:The sera from 196 patients were measured by FQ PCR.Results:In the 112 HBeAg(+)/HBeAb(-) samples,the FQ PCR results were all positive,with 1.08×10 7/ml of HBV DNA on the average.In the 68 HBeAg(-)/HBeAb(+) samples,the positive rate was 66.2 % with a duplication amount of 6.12×10\+5/ml on the average.In the 16 HBeAg(-)/HBeAb(-) samples,the duplication amount was 2.72×10\+4/ml.Conclusions:FQ PCR can ensure the accurate quantity.It is a useful method in understanding HBV duplication and evaluating the therapeutic effect.更多还原