【摘要】 背景与目的:BRM释介素在临床上对一些肿瘤有一定疗效,但其机理仍不清楚。本研究旨在探讨中药复方胶囊BRM释介素诱导人神经胶质瘤细胞株SHG-44,人乳腺癌细胞株MCF-7的凋亡作用,及其抗肿瘤作用的机理。方法:运用MTT法检测BRM释介素体外对人癌细胞株的抑制作用;应用电镜、AnnexinV-FITC/PI双染流式细胞仪分析、琼脂糖凝胶电泳观察BRM释介素诱导SHG-44,MCF-7细胞发生凋亡的作用。结果:BRM释介素体外对SHG-44,MCF-7,PANC1细胞具有明显的抑制作用,IC50值分别为0.299mg/ml,1.853mg/ml和9.416mg/ml。SHG-44和MCF-7在BRM水提液(2.5mg)作用下发生细胞凋亡,表现为细胞固缩、核染色质靠边,出现马蹄形,半月形。AnnexinV-FITC/PI流式细胞仪分析,BRM(0.625～2.5mg)作用14～48h,凋亡细胞明显增多。BRM水提液(1.25～5mg)作用24或48h,细胞DNA裂解片段呈典型的梯带。结论:BRM释介素的抗肿瘤活性与诱导细胞凋亡有关。更多还原

【Abstract】 BACKGROUND &OBJECTIVE: It had been observed that BRM-SJS had an ti tumor effect in our clinical practice. This study was designed to investigate th e antitumor activity of BRM-SJS,and mechanism of its action. METHODS: In vitro antitumor experiments with MTT method,meanwhile cell morphology,flow cytometry,a nd agarose gel electrophoresis were performed for determining apoptosis in sever al tumor cell lines. RESULTS: BRM-SJS had antitumor effects on human Suzhou hum an glioma(SHG-44),breast carcinoma(MCF-7),and human pancreas carcinoma(PANC1) in vitro,the IC50 values of BRM-SJS were 0.299 mg/ml,1.853 mg/ml and 9.416 mg/m l respectively. At the 2.5 mg of BRM-SJS on SHG-44 and MCF-7,marked morpholog ical changes, including cell shrinkage and condensation of chromosomes,were obse rved with electric microscope. The increase of apoptosis in SHG-44 and MCF-7 c ells treated with BRM-SJS extracts 0.625-2.5 mg for 14-48 h was observed by A nnexin-V/PI flow cytometry analysis. Agarose gel electrophoresis of DNA from SH G-44 and MCF-7 cells treated with BRM-SJS extracts 1.25-5 mg for 24 h or 48 h showed marked DNA Ladder pattern. CONCLUSION: Antitumor activity of BRM-SJS m ay be related with inducement of apoptosis of tumor cells.更多还原