【摘要】 背景与目的:鼻咽癌致病的分子机理至今仍不清楚。有研究表明ΔNp63以显性失活的方式抑制p53的反式激活,并与鳞状细胞的恶性增生与失分化有关。本研究旨在探讨ΔNp63蛋白在鼻咽癌组织中的表达及意义。方法:采用免疫组织化学方法检测60例鼻咽癌组织、37例癌旁粘膜及30例鼻咽粘膜慢性炎症组织中ΔNp63蛋白的表达。60例鼻咽癌的类型为角化性鳞癌3例,非角化性癌57例,其中后者包括未分化癌12例。结果:全部60例鼻咽癌组织ΔNp63染色呈阳性表达。角化性鳞癌均呈弱阳性表达,非角化性癌的强阳性表达率为66.67%(38/57),未分化癌的强阳性表达率为83.33%(10/12)。慢性炎症组织及癌旁粘膜仅基底层及上基层细胞阳性。结论:ΔNp63蛋白与细胞的恶性增生密切相关;ΔNp63蛋白表达在幼稚或间变鳞状细胞中有明显的倾向性,是诊断间变鳞状细胞有价值的标志物。更多还原

【Abstract】 BACKGROUND & OBJECTIVE:The molecular etiology of nasopharyngeal c arcinoma is currently unknown. It was reported that ΔNp63 is dominant-negative toward transactivation of p53, and associated with the malignant hyperplasia an d dedifferentiation of the squamous cells. This study was designed to investigat e the expression and significance of ΔNp63 protein in NPC tissues. METHODS: Imm unohistochemical method was used to determine the expression of ΔNp63 in 60 cas es of NPC, 37 cases of nearby mucosa of the carcinoma, and 30 cases of chronic inflammation of nasopharyngeal mucosa. The classification of the 60 NPC cases we re as follows: 3 cases of keratinizing squamous cell carcinomas, 57 cases of non keratinizing carcinomas including 12 cases of undifferentiated carcinomas. RESUL TS:All 60 NPC cases showed ΔNp63 positive reaction. Keratinizing squamous cell carcinomas showed weakly positive expression; 66.67% (38/57) of nonkeratinizi ng carcinomas showed strongly positive reaction, and 83.33% (10/12) of undiffe rentiated carcinomas showed strongly positive reaction. Chronic inflammation muc osas and cancer nearby mucosas showed positive reaction only in their basal and superbasal cells. CONCLUSION: ΔNp63 protein was found to be closely associated with cell malignant hyperplasia. ΔNp63 protein has distinct tendency to the inf antile or anaplastic squamous cells, and ΔNp63 protein is a valuable diagnostic marker for anaplastic squamous cells.更多还原