【摘要】 背景与目的:目前尚缺乏有效评价喉鳞癌预后的理想指标。本研究旨在探讨喉鳞状细胞癌(laryngealsquamouscellcarcinoma,LSCC)中p73、PTEN(phosphatesandtensionhomologdeletedonchromosometen,PTEN)基因的表达与患者临床、病理参数和预后的关系。方法:采用免疫组化SABC技术检测65例喉鳞癌组织及23例癌旁组织中p73、PTEN基因的表达情况。结果:P73、PTEN蛋白在65例喉鳞癌组织及23例癌旁组织中的表达存在显著性差异(P<0.05),P73蛋白阳性率分别为58.5%(38/65)与17.4%(4/23),PTEN蛋白阳性率分别为49.2%(32/65)与95.7%(22/23)。65例喉鳞癌组织中,P73蛋白在TNM分期Ⅲ～Ⅳ期、远处转移及复发患者中的表达水平较Ⅰ～Ⅱ期、无转移及初发患者高(P<0.05);PTEN蛋白的表达在TNM分期Ⅰ～Ⅱ期、高分化癌和无颈淋巴结转移及远处转移患者中的表田慎之,等.喉鳞状细胞癌中p7达水平较Ⅲ～Ⅳ期、中低分化和有转移患者高(P<0.05)。术后生存5年以上者的PTEN蛋白阳性率(66.7%)较生存时间不超过5年者(27.3%)高(P<0.05)。喉鳞癌组织中p73、PTEN基因的表达未见显著性相关(P>0.05)。结论:PTEN蛋白的表达对预测喉鳞癌的预后有一定参考价值。更多还原

【Abstract】 BACKGROUND &OBJECTIVE:There are few ideal predictors used to evalu ate the prognosis of laryngeal squamous cell carcinoma (LSCC). This study was de signed to investigate the expression of p73 and PTEN (phosphates and tension hom olog deleted on chromosome ten) and their association with clinical, histologic characteristics and prognosis in LSCC. METHODS: p73 protein and PTEN protein wer e examined by using immunochistochemical SABC staining method in 65 cases of LSC C and in 23 cases of para-tumor tissues. RESULTS: p73 protein and PTEN protein in LSCC showed positive expression of about 58.5%(38/65) and 49.2%(32/65), com pared to para-tumor tissues of about 17.4%(4/23) and 95.7%(22/23) with statis tical significance (P< 0.05). p73 protein positive expression showed stronger in stage ⅢⅣof LSCC than that in stage ⅠⅡ(P< 0.05); it more often appeared in recurrent cases than in primary cases (P< 0.05). And p73 protein positive exp ression with distant metastasis was stronger than that in LSCC without distant m etastasis (P< 0.05). PTEN protein positive expression was stronger in stage Ⅰ Ⅱof LSCC than that in stage ⅢⅣ(P< 0.05); PTEN protein positive expression a ppears less frequently in poor differentiation of LSCC, compared to well/moderat e differentiation (P< 0.05); PTEN positive expression in cases with cervical lym ph node metastasis and distant metastasis was lower than that in those without m etastasis (P< 0.05). PTEN expression showed significantly stronger in the patien ts whose survival time over 5 years (66.7%) than in the patients whose survival time was less than 5 years (27.3%) (P< 0.05). CONCLUSION:PTEN positive express ion may be useful for predicting the prognosis of LSCC.更多还原