【摘要】 采用叶碟法、夹毒法、浸叶法、点滴法和注射法 ,测定了瑞香狼毒 (StellerachamaejasmeL .)根乙醇提取物 (简称SCEE)对菜粉蝶 (PierisrapaeL .)的多种生物活性。结果表明 :SCEE对菜粉蝶幼虫具有很强的拒食、胃毒、生长抑制及毒杀作用 ,但对菜粉蝶幼虫触杀作用差。SCEE对菜粉蝶 3、4、5龄幼虫 2 4h非选择性拒食作用的AFC50 (拒食中浓度 )值分别为 2 6.2 0、88.64、3 81.2 8μg/ml;对菜粉蝶 5龄幼虫 12、2 4、48h胃毒作用的LD50 分别为 5 6.97、2 2 .42、12 .3 2 μg /头 ;当处理浓度为 5mg/ml时 ,2d后的体重只有对照的 2 /3 ,7d后幼虫校正死亡率为 10 0 % ;12、2 4、3 6、48h对昆虫毒杀作用的LD50 分别为 9.72、2 .68、1.60、1.0 8μg/头 ;对 3、4、5龄幼虫触杀作用的LD50 分别为 0 .3 7、2 .10、5 2 .48μg/头更多还原

【Abstract】 Bioactivity of SCEE (extraction of root of Stellera chamaejasme with ethanol by dip) against Pieris rapae was tested by disc leaf ,sandwich,dip leaf,topical applications,and injection methods. The results showed that SCEE possessed strong antifeedant,stomach poison,and development inhibiting effects,while weak contact toxicity effect AFC 50 (content causing 50% antifeedant rate) of non-choice of SCEE to the third,fourth,fifth instar larvae were 26.20,88.64,and 381.28?μg/ml within 24 hours respectively;LD 50(lethal dosage causing 50% mortality)of stomach poison of SCEE to the fifth instar larvae were 56.97,22.42,and 12.32?μg/larvae within 12,24,and 48 h respectively; treatment of concentration with SCEE at 5 mg/ml for the fifth instar larvae ,the weight of them was two-third of check within 2 days and the corrected mortality was 100% within 7 days after treatment;LD 50 of lethal effect of SCEE was 9.72,2.68,1.60,1.08?μg/larvae within 12,24,36 and 48 h respectively;LD 50 of contact toxicity of SCEE to the third,fourth,fifth instar larvae were 0.37,2.10,52.48?μg/larvae.更多还原