【摘要】 目的 :从组织形态学角度观察、比较新鲜羊膜 (Freshamnioticmembrane ,FSAM )、冻干羊膜 (Frozenamnioticmembrane ,FZAM )、羊膜细胞外基质 (Amnioticextracellularmatrix ,AECM )的结构特点。方法 :采用目前国际公认的羊膜制备与保存方法 ,制备FSAM、FZAM、AECM ,分别经HE、PAS组织染色、透射电子显微镜、扫描电子显微镜检查 ,观察并比较它们的形态与结构特点。结果 :FSAM、FZAM在光镜和电镜下均与正常球结膜组织结构相近 ,两者形态上的主要差别表现在上皮细胞的结构变化上。而AECM结构较为独特 ,无细胞结构 ,其主要成分为胶原纤维、网状纤维和基质。结论 :从组织结构特点分析 ,FSAM、FZAM、AECM是目前较为理想的结膜替代材料并可有效地用于角、结膜表面重建 ;同时 ,AECM作为一种极为特殊的细胞外基质 ,有重要的研究与应用价值。更多还原

【Abstract】 Objective:To compare the structural characteristics of fresh amniotic membrane(FSAM),frozen amniotic membrane(FZAM) and amniotic extracellular membrane(AECM) on morphology. Methods:All the pieces of FSAM,FZAM and AECM were photographed and examined by HE and PAS stains under light microscope(LM),transmission electron microscope(TEM) and scanning electron microscope(SEM). Results:Both FSAM and FZAM were similar to conjunctival tissues under the LM,TEM and SEM.The major difference between FSAM and FZAM was structural changes in epithelial layer.But AECM was special,with cell-less characteristics and mainly was collagen fibers,reticular fibers and ground substance. Conclusions:FSAM,FZAM and AECM are ideal conjunctival succedaneum in the plastic surgery of conjunctiva,and could be used as a graft to reconstruct ocular surface.AECM,as a sort of special ECM,has expansive application in the future.更多还原