【摘要】 目的 :探讨穿透性角膜移植的供眼角膜内皮细胞变异情况及影响其变异的因素。方法 :用角膜内皮显微镜对穿透性角膜移植 44只眼的供眼及术后移植片行内皮细胞摄相分析。并用超声角膜测厚仪测量供眼角膜及术后移植片厚度。结果 :术后 2～ 6周、 3～ 6月、 7～ 12月及 1 5～ 2年的内皮细胞丧失率分别为 12 98%、 2 2 2 3 %、 2 7 2 6%及 2 9 78%。平均细胞面积由术前的 44 5 13 μm2 增加到术后 1 5～ 2年的 65 4 42 μm2 ,细胞大小变异系数 (CV值 )由 43 2 4%增加到 64 76% ,六角形细胞比例由 45 2 6%下降到 2 7 18%。单纯穿透性移植组与穿透性移植联合手术组术后 1 5年～ 2年的细胞丧失率、平均细胞面积、CV值和六角形细胞比例均有显著性差异 (P <0 0 5 ) ,但移植片厚度无显著性差异 (P >0 0 5 )。结论 :穿透性角膜移植后供眼角膜内皮细胞密度逐渐减少 ,平均细胞面积和CV值均渐增大 ,六角形细胞比例渐变小。穿透性角膜移植联合手术比单纯穿透性移植的术后内皮细胞丧失率较高 ,平均细胞面积和CV值增大较显著 ,六角形细胞比例下降较大但移植片厚度无显著性差异。更多还原

【Abstract】 Objective:To study the variation of donor corneal endothelium following penetrating keratoplasty. Methods:Specular microscopy of donor corneal endothelium was performed on 43 patients (44 eyes) who underwent penetrating keratoplasty.The thickness of donor cornea and grafts were measured. Results:The loss rates of the corneal endothelium were 12 98%,22 23%,27 26% and 29 78% in postoperative 2～6 weeks,3～6 months,7～12 months and 1 5～2 years,respectively.The average cell area of graft increased from 445 13μm 2 of preoperation to 654 42μm 2 in 1 5～2 years postoperatively.The coefficient of variation (CV) of cell size increased from 43 24% to 64 76%.The hexagonality of donor corneal endothelium decreased from 45 26% to 27 18% after operation 1 5～2 years. Conclusions:After penetrating keratoplasty,the density of donor corneal endothelium would decrease gradually,the average cell area and CV increase and the hexagonality decrease.更多还原