【摘要】 目的:观察左旋四氢巴马汀(L-tetrahydropalmatine,L-THP)在脑缺血再灌注时对凋亡相关基因蛋白表达的影响。方法:在建立大鼠急性全脑缺血再灌注模型基础上,采用免疫组织化学法、原位末端标记法,分别检测假手术组、脑缺血再灌注组及 L-THP 治疗组:海马 CAl 区 Caspase-3、Fas、c-Fos、c-Myc、P53阳性细胞及凋亡细胞数。结果:脑缺血再灌注时,Caspase-3、Fas、c-Fos、C-Myc、P-53阳性细胞及细胞凋亡数均增加,与假手术组比较差异有显著性(P<0.01),L-THP 可减少这几项参数,L-THP 治疗组与脑缺血再灌注组比较差异亦有显著性(P<0.05,P<0.01)。结论:在脑缺血再灌注细胞凋亡过程中,L-THP 可通过抑制上述凋亡相关基因蛋白的表达,减少神经元凋亡,对脑缺血损伤起保护作用。更多还原

【Abstract】 Objective:To study the effects of L-tetrahydropalmatine(L-THP) on the expression of gene protein related to neuronal apopto- sis after cerebral ischemia/reperfusion in rats.Methods:Pulsinelli’s 4 blood vessels occlusion model was established to induce cerebral ischemia/reperfusion.Three groups were divided:sham-operation group(SO),ischemia/reperfusion group (IR) and L-THP treated group(THP).Changes in protein expression of Caspase-3,Fas,c-Fos,c-Myc,P53 were evaluated by immunohistochemical method. The number of neuronal apoptosis was assessed by in situ end labeling.Results:The positive cells of Caspase-3,Fas,c-Fos,c-Myc, P53 in the IR group was much more than those in the SO group ((P<0.01 ).Compared with IR group,the positive cells were much reduced than those in THP group (P<0.05,P<0.01).Conclusion.After cerebral ischemia/reperfusion,L-THP can protect neu- rons from ischemia and injury by suppressing the protein expression and blocking the neuronal apoptosis.更多还原