【摘要】 目的　观察顺铂 (DDP)对人肺癌SPCA 1细胞端粒酶活性的影响。方法　不同浓度的DDP处理体外培养的人肺癌SP CA 1细胞 72h ,非放射性标记的改良TRAP法测定端粒酶活性 ,MTT法测定细胞增殖抑制 ,透射电镜、流式细胞仪观察细胞凋亡。结果　DDP可抑制SPCA 1细胞端粒酶活性 ,随DDP浓度增大 ,端粒酶活性抑制增强。MTT结果显示DDP抑制SP CA 1细胞增殖且抑制率有浓度依赖性。透射电镜可观察到典型的细胞凋亡形态学 ,流式细胞仪结果表明DDP诱导SPCA 1细胞凋亡在一定浓度范围内呈剂量依赖性。结论　端粒酶活性的抑制可能是DDP发挥抗肺癌活性的作用机制之一 ,端粒酶活性可作为评价DDP抗肺癌化疗效果的指标之一。更多还原

【Abstract】 ObjectiveTo observe the effects of cisplatin(DDP) on telomerase activity in human lung cancer cells. MethodsSPCA-1 was incubated with different concentrations of DDP for 72 hours. Telomerase activity was measured by using nonradioactive TRAP method. The cytotoxicity of DDP on SPCA-1 cells was analysed with MTT assay. Cell apoptosis was evaluated by electron microscopy and flow cytometry. ResultsOwing to effect of DDP, telomerase activity of SPCA-1 cells was decreased, this phenomenon showed a dose dependence.According to the result of MTT assay , DDP could inhibit cellproliferation.The inhibitory rate depended on DDP concentration.Cell apoptosis with clear morphological changes was observed under electron microscopy. Data from flow cytometry also proved that cell apoptosis was induced by DDP and was dose dependent. ConclusionThe down-regulation of telomerase activity probably was one of the important mechanism of killing lung cancer cells by DDP. Telomerase activity may become a marker used for judging the chemotherapeutic effect of DDP in treating lung cancer.更多还原