【摘要】 目的　探讨EB病毒LMP1对早期鼻咽癌中瘤细胞分化的影响。方法　收集 31例早期鼻咽非角化性癌活检组织。采用DAKO公司的PNA探针和PNA原位杂交试剂盒检测EB病毒早期RNAs (EBERs)。应用免疫组化法检测LMP1,α catenin ,β catenin ,γ catenin以及高、低分子量细胞角蛋白。结果　 31例癌组织中均有相当数量的瘤细胞呈EBERs阳性 ,其中19例表达LMP1( 6 1.2 9% )。LMP1阳性组γ catenin的表达百分率 ( 5 3 .2 5± 34.12 % )明显低于LMP1阴性组 ( 80 .42±15 .77% ,P <0 .0 1)。γ catenin的表达与低分子量细胞角蛋白的表达间呈正相关 (r=0 .44 0 ,P <0 .0 5 )。α catenin ,β catenin和γ catenin的表达相互间呈两两正相关。结论　鼻咽癌中瘤细胞的LMP1能下调γ catenin的表达 ,从而抑制瘤细胞的分化。更多还原

【Abstract】 Objective To investigate the effects of LMP1 (Latent Membrane Protein) expression on neoplastic cell differentiation of early nasopharyngeal carcinomas. Methods Thirty-one biopsy specimens of early nasopharyngeal non-keratinizing carcinoma were collected. The Epstein-Barr virus early RNAs (EBERs) was detected by use of DAKO PNA Probe (Y5200) and PNA ISH Detection Kit (K5201). The LMP1, α-catenin, β-catenin, γ-catenin, high-molecular and low-molecular weight cytokeratins were detected by immunohistochemistry. Results All 31 carcinoma specimens studied showed a considerable number of neoplastic cells with EBERs expressed positivity, and 19 out of 31 cases (61.29%) expressed LMP1. The mean percentage of γ-catenin expression in LMP1 positive group (53.25±34.12% ) was significantly lower than that (80.42±15.77%) in LMP1 negative group, P <0.01. The γ-catenin expression was positively correlated with the expression of low molecular weight cytokeratin ( r=0.440, P <0.05). There were positive correlations for the expression of α-catenin, β-catenin and γ-catenin in between. Conclusion The LMP1 could down-regulate the expression of γ-catenin and thus inhibit the neoplastic cell differentiation of early nasopharyngeal carcinoma.更多还原