【摘要】 目的 :比较补肾、健脾、益气、活血法对衰老细胞增殖的调控作用。方法 :采用含药血清对衰老的人胚肺二倍体成纤维细胞 2BS细胞株进行处理 ,观察四种中药对细胞寿命、细胞生长曲线、代谢活力 (MTT法 )以及衰老细胞生物学标志衰老相关半乳糖苷酶活力的影响。结果 :补肾和益气方药能够明显延长衰老细胞的传代次数 ,促进细胞增殖 ,使细胞MTT的还原量明显升高 ,抑制细胞衰老相关半乳糖苷酶 (SA β Gal)活力。而健脾、活血方药对细胞增殖的促进作用不明显。 结论 :细胞衰老时增殖变慢 ,活力降低 ,衰老相关半乳糖苷酶活力增高。补肾、益气中药能改善上述衰老变化 ,健脾、活血药作用不明显。更多还原

【Abstract】 Objective: To compare the effect of Sheng tonifying, Qi benefiting, Pi invigorating and Xue activating recipes on the regulation of proliferation in senescence cell. Methods: Four kinds of drug sera of Sheng tonifying, Qi benefiting, Pi invigorating and Xue activating recipes were used to treat the human diploid fibroblast cells 2BS. The proliferative life spans of the cells, the cell growth curves, the cell metabolic activity (MTT assay) and the biomarker that identifies the senescent human cells i.e the senescence associated β galacto sidase (SA β Gal) activity were observed. Results: Both sheng tonifying recipe and Qi benifiting recipe could significantly extent the prolifer ative doublings of 2BS cells, improve the cell proliferative ability and MTT reduction, and decrease the SA β Gal activity. But the effect of pi invigorating recipe and xue circulation activating recipe were not obvious.更多还原