【摘要】 目的 探讨PBV220及PJW2载体构建的rhIL-18重组质粒的IL-18表达效率。方法 将构建的PBV220/rhIL-18重组质粒中的rhIL-18酶切、克隆到表达载体PJW2中,经PCR产物电泳及测序鉴定,热诱导后在大肠杆菌中表达。结果 PJW2/rhIL-18表达的重组人IL-18占菌体总蛋白质的20％,而PBV220/IL-18重组质粒表达的重组人IL-18占菌体总蛋白质的16％。两者表达的蛋白质分子量约为18KD,与预期分子量相符。结论 PJW2载体rhIL-18表达效率高于PBV220,为下一步的纯化及提高蛋白产量打下了基础。更多还原

【Abstract】 Objictive To realize the expression level of rhIL - 18 that expressed by the recombinant plasmid PJW2 / IL - 18 and PBV220 / IL - 18. Methods The mature human interleukin - 18 gene picked from the recombinant plasmid PBV220 / IL - 18 which have been constructed before was cloned into thermo - sensitive vector PJW2 and it’s sequence was identified. The E. coli DH5α harbouring the two kinds of constructs expressed the same recombinant protein - rhIL - 18. Results the expression rate of PJW2 / IL - 18 was 20% while that of PBV220 / IL - 18 was 16% . Conclusion the expression level of PJW2 / IL - 18 was higher than that of PBV220/IL-18.更多还原