【摘要】 目的　了解α1-抗糜蛋白酶 (AACT)基因Isehara - 1、Isehara - 2突变型与昆明儿童哮喘发生是否具有相关性。方法　应用聚合酶链反应 (PCR)技术、基因测序方法 ,对 30例昆明市汉族哮喘儿童及 2 0例正常儿童α1-抗糜蛋白酶基因外显子 (exon)V进行检测。结果　所有研究对象均未发现α1-抗糜蛋白酶基因Isehara - 1、Isehara - 2突变型。结论　α1-抗糜蛋白酶基因Isehara- 1、Isehara - 2突变型与 30例昆明市汉族儿童哮喘发病无相关关系。更多还原

【Abstract】 Objective: To understand α 1-antichymotrypsin gene Isehara-1?Isehara-2 mutations of children asthma in Kun′ming. Methods: 30 patients with children asthma and 20 controls were tested for exonV of α 1-antichymotrypsin by PCR and gene sequence to analyze AACT encoding gene. Results: Isehara-1?Isehara-2 mutations of the AACT gene were not found in any of the subjects.Conclusion: There is no relationship between α1-antichymotrypsin gene mutations including Isehara-1?Isehara-2 and 30 han asthmatic children in Kunming.更多还原