【摘要】 目的 建立肝炎灵注射液中苦参碱含量的HPLC测定方法。方法 采用μBondapak^TMNH₂柱（4.6 mm×300mm,5μm）,甲醇-水（15:85）为流动相,流速为1.0 mL·min^-1,检测波长219 nm,柱温为30℃,进样量20μL。结果 在1.0 μg～500.0μg·mL^-1范围内苦参碱浓度与峰面积呈良好的线性关系（r=0.9999）,平均回收率为98.3～100.9％,RSD<2％。结论 该方法简单,快速,重复性好。更多还原

【Abstract】 Objective To establish the method for the determination of matrine in Ganyanlin injection by high performance liquid chromatography （HPLC）. Methods A μBondapak^TM NH₂（4.6 mm×300 mm 5 μm） column was selected as separation column at 30℃, injection volume was 20 μL and matrine was isocratically eluted by methanol-water（15: 85）at flow rate of 1.0 mL·min^-1. The peak of matrine was monitored at UV 219 nm. Results There was a good linear relation- ship between the concentration and the peak area of matrine in range of 1.0～500.0 μg·mL^-1. The average recovery was 98.3～100.9% and RSD<2%. Conclusion The method is simple, rapid and reproducible.更多还原